info:eu-repo/semantics/article
Immunization with Neospora caninum profilin induces limited protection and a regulatory T-cell response in mice
Fecha
2015-11Registro en:
Mansilla, Florencia Celeste; Quintana, Maria Eugenia; Langellotti, Cecilia Ana; Wilda, Maximiliano; Martinez, Andrea; et al.; Immunization with Neospora caninum profilin induces limited protection and a regulatory T-cell response in mice; Academic Press Inc Elsevier Science; Experimental Parasitology; 160; 11-2015; 1-10
0014-4894
CONICET Digital
CONICET
Autor
Mansilla, Florencia Celeste
Quintana, Maria Eugenia
Langellotti, Cecilia Ana
Wilda, Maximiliano
Martinez, Andrea
Fonzo, Adriana
Moore, Dadin Prando
Cardoso, Nancy Patricia
Capozzo, Alejandra Victoria
Resumen
Profilins are actin-binding proteins that regulate the polymerization of actin filaments. In apicomplexan parasites, they are essential for invasion. Profilins also trigger the immune response of the host by activating TLRs on dendritic cells (DCs), inducing the production of pro-inflammatory cytokines. In this study we characterized for the first time the immune response and protection elicited by a vaccine based on Neospora caninum profilin in mice. Groups of eight BALB/c mice received either two doses of a recombinant N. caninum profilin expressed in E. coli. (rNcPRO) or PBS, both formulated with an aqueous soy-based adjuvant enriched in TLR-agonists. Specific anti-profilin antibodies were detected in rNcPRO-vaccinated animals, mainly IgM and IgG3, which were consumed after infection. Splenocytes from rNcPRO-immunized animals proliferated after an in vitro stimulation with rNcPRO before and after challenge. An impairment of the cellular response was observed in NcPRO vaccinated and infected mice following an in vitro stimulation with native antigens of N. caninum, related to an increase in the percentage of CD4+CD25+FoxP3+. Two out of five rNcPRO-vaccinated challenged mice were protected; they were negative for parasite DNA in the brain and showed no histopathological lesions, which were found in all PBSvaccinated animals. As a whole, our results provide evidence of a regulatory response elicited by immunization with rNcPRO, and suggest a role of profilin in the modulation and/or evasion of immune responses against N. caninum.