info:eu-repo/semantics/article
Emotional stress induces structural plasticity in Bergmann glial cells via an AC5–CPEB3–GluA1 pathway
Fecha
2020-04Registro en:
Bender, Crhistian Luis; Sun, Xingxing; Farooq, Muhammad; Yang, Qian; Davison, Caroline; et al.; Emotional stress induces structural plasticity in Bergmann glial cells via an AC5–CPEB3–GluA1 pathway; Society for Neuroscience; Journal of Neuroscience; 40; 17; 4-2020; 3374-3384
0270-6474
CONICET Digital
CONICET
Autor
Bender, Crhistian Luis
Sun, Xingxing
Farooq, Muhammad
Yang, Qian
Davison, Caroline
Maroteaux, Matthieu
Huang, Yi Shuian
Ishikawa, Yoshihiro
Liu, Siqiong June
Resumen
Stress alters brain function by modifying the structure and function of neurons and astrocytes. The fine processes of astrocytes are critical for the clearance of neurotransmitters during synaptic transmission. Thus, experience-dependent remodeling of glial processes is anticipated to alter the output of neural circuits. However, the molecular mechanisms that underlie glial structural plasticity are not known. Here we show that a single exposure of male and female mice to an acute stress produced a long-lasting retraction of the lateral processes of cerebellar Bergmann glial cells. These cells express the GluA1 subunit of AMPA-type glutamate receptors, and GluA1 knockdown is known to shorten the length of glial processes. We found that stress reduced the level of GluA1 protein and AMPA receptor-mediated currents in Bergmann glial cells, and these effects were absent in mice devoid of CPEB3, a protein that binds to GluA1 mRNA and regulates GluA1 protein synthesis. Administration of a b-adrenergic receptor blocker attenuated the reduction in GluA1, and deletion of adenylate cyclase 5 prevented GluA1 suppression. Therefore, stress suppresses GluA1 protein synthesis via an adrenergic/adenylyl cyclase/CPEB3 pathway, and reduces the length of astrocyte lateral processes. Our results identify a novel mechanism for GluA1 subunit plasticity in non-neuronal cells and suggest a previously unappreciated role for AMPA receptors in stress-induced astrocytic remodeling.