info:eu-repo/semantics/article
Characterization of the molecular changes associated with the overexpression of a novel epithelial cadherin splice variant mRNA in a breast cancer model using proteomics and bioinformatics approaches: identification of changes in cell metabolism and an in
Fecha
2019-05Registro en:
Rosso, Marina; Lapyckyj, Lara; Besso, María José; Monje, Marta; Reventós, Jaume; et al.; Characterization of the molecular changes associated with the overexpression of a novel epithelial cadherin splice variant mRNA in a breast cancer model using proteomics and bioinformatics approaches: identification of changes in cell metabolism and an in; Springer; Cancer and Metabolism; 7; 1; 5-2019
2049-3002
CONICET Digital
CONICET
Autor
Rosso, Marina
Lapyckyj, Lara
Besso, María José
Monje, Marta
Reventós, Jaume
Canals, Francesc
Quevedo Cuenca, Jorge Oswaldo
Matos, María Laura
Vazquez, Monica Hebe
Resumen
Breast cancer (BC) is the most common female cancer and the leading cause of cancer death in women worldwide. Alterations in epithelial cadherin (E-cadherin) expression and functions are associated to BC, but the underlying molecular mechanisms have not been fully elucidated. We have previously reported a novel human E-cadherin splice variant (E-cadherin variant) mRNA. Stable transfectants in MCF-7 human BC cells (MCF7Ecadvar) depicted fibroblast-like cell morphology, E-cadherin wild-type downregulation, and other molecular changes characteristic of the epithelial-to-mesenchymal transition process, reduced cell-cell adhesion, and increased cell migration and invasion. In this study, a two-dimensional differential gel electrophoresis (2D-DIGE) combined with mass spectrometry (MS) protein identification and bioinformatics analyses were done to characterize biological processes and canonical pathways affected by E-cadherin variant expression.