info:eu-repo/semantics/article
The polyhydroxyalkanoate genes of a stress resistant Antarctic Pseudomonas are situated within a genomic island
Fecha
2007-11Registro en:
Ayub, Nicolás Daniel; Pettinari, María Julia; Mendez, Beatriz Silvia; López, Nancy Irene; The polyhydroxyalkanoate genes of a stress resistant Antarctic Pseudomonas are situated within a genomic island; Academic Press Inc Elsevier Science; Plasmid; 58; 3; 11-2007; 240-248
0147-619X
CONICET Digital
CONICET
Autor
Ayub, Nicolás Daniel
Pettinari, María Julia
Mendez, Beatriz Silvia
López, Nancy Irene
Resumen
Pseudomonas sp. 14-3 is an Antarctic bacterium that shows high stress resistance in association with high polyhydroxybutyrate (PHB) production. In this paper genes involved in PHB biosynthesis (phaRBAC) were found within a genomic island named pha-GI. Numerous mobile elements or proteins associated with them, such as an integrase, insertion sequences, a bacterial group II intron, a complete Type I protein secretion system and IncP plasmid-related proteins were detected among the 28 ORFs identified in this large genetic element (32.3 kb). The G + C distribution was not homogeneous, likely reflecting a mosaic structure that contains regions from diverse origins. pha-GI has strong similarities with genomic islands found in diverse Proteobacteria, including Burkholderiales species and Azotobacter vinelandii. The G + C content, phylogeny inference and codon usage analysis showed that the phaBAC cluster itself has a complex mosaic structure and indicated that the phaB and phaC genes were acquired by horizontal transfer, probably derived from Burkholderiales. These results describe for the first time a pha cluster located within a genomic island, and suggest that horizontal transfer of pha genes is a mechanism of adaptability to stress conditions such as those found in the extreme Antarctic environment.