info:eu-repo/semantics/article
Biorefining via solid-state fermentation of rice and sunflower by-products employing novel monosporic strains from Pleurotus sapidus
Fecha
2019-10Registro en:
Postemsky, Pablo Daniel; Bidegain, Maximiliano Andrés; Lluberas Nuñez, María Gabriela; Lopretti Correa, Mary Isabel; Bonifacino, Silvana; et al.; Biorefining via solid-state fermentation of rice and sunflower by-products employing novel monosporic strains from Pleurotus sapidus; Elsevier; Bioresource Technology; 289; 10-2019; 121692
0960-8524
CONICET Digital
CONICET
Autor
Postemsky, Pablo Daniel
Bidegain, Maximiliano Andrés
Lluberas Nuñez, María Gabriela
Lopretti Correa, Mary Isabel
Bonifacino, Silvana
Landache, María Inés
Zygadlo, Julio Alberto
Fernandez Lahore, Marcelo
Omarini, Alejandra Beatriz
Resumen
Pleurotus sapidus monokaryotic strains (Mk) were screened as a novel source of mycelia to valorize rice straw (RS), rice husks (RH) and sunflower seed hulls (SSH) into value-added products through solid-state fermentation (SSF). P. sapidus Dk3174 basidiospores were cultured in the presence of Remazol Brillant Blue R for strain selection, revealing the ligninolytic ability of emerging colonies. Further screening demonstrated the intraspecific variability in dye degradation and enzyme production of 63 strains. Growth rate, biomass and enzyme production in plates containing RS, RH or SSH pointed at MkP6 as a suitable strain for pilot-scale SSF. MkP6 presented a similar laccase profile as the parental Dk3174, being greater in pasteurized substrates (300?1200 U/Kg) than in sterilized substrates (30?250 U/Kg). Peroxidase represented 25% of the total ligninolytic activity measured. The SSH fermented biomass with MkP6 obtained good yields of nanocellulose (67%) and the saccharide release for ethanol production increased by 3?4 times.