info:eu-repo/semantics/article
A striking common O-linked N-acetylglucosaminyl moiety between cruzipain and myosin
Fecha
2011-07Registro en:
Acosta, Diana Maria; Soprano, Luciana Lía; Ferrero, Maximiliano Ruben; Landoni, Malena; Esteva, Mónica Inés; et al.; A striking common O-linked N-acetylglucosaminyl moiety between cruzipain and myosin; Wiley Blackwell Publishing, Inc; Parasite Immunology; 33; 7; 7-2011; 363-370
0141-9838
CONICET Digital
CONICET
Autor
Acosta, Diana Maria
Soprano, Luciana Lía
Ferrero, Maximiliano Ruben
Landoni, Malena
Esteva, Mónica Inés
Couto, Alicia Susana
Duschak, Vilma Gladys
Resumen
Single units of O-linked N-acetylglucosamine (GlcNAc), usually components of nuclear and cytoplasmatic proteins, are present at the C-terminal domain of cruzipain (Cz), a lysosomal major antigen from Trypanosoma cruzi. On the other hand, antibodies directed against some self-antigens like myosin are associated with Chagas heart disease. The participation of O-GlcNAc moieties in the molecular antigenicity of Cz was determined using GlcNAc linked to aprotinin by ELISA. The immune cross-reactivity between Cz and myosin is mainly focused in the C-T domain. ELISA inhibition assays using rabbit sera specific for Cz and C-T in conjunction with immune-gold electron microscopy analysis of heart tissues from mice immunized with C-T confronted with polyclonal rabbit sera specific for Cz and C-T prior and after myosin adsorption provided evidence which indicates that O-GlcNAc moieties constitute a common epitope between Cz and either myosin or other cardiac O-GlcNAc-containing proteins, showing a new insight into the molecular immune pathogenesis of Chagas heart disease.