info:eu-repo/semantics/article
Identification and characterization of a new potyvirus infecting cucurbits
Fecha
2018-03Registro en:
Perotto, Maria Cecilia; Pozzi, Elizabeth Alicia; Celli, Marcos Giovani; Luciani, Cecilia Elizabeth; Mitidieri, Mariel Silvina; et al.; Identification and characterization of a new potyvirus infecting cucurbits; Springer Wien; Archives of Virology; 163; 3; 3-2018; 719-724
0304-8608
1432-8798
CONICET Digital
CONICET
Autor
Perotto, Maria Cecilia
Pozzi, Elizabeth Alicia
Celli, Marcos Giovani
Luciani, Cecilia Elizabeth
Mitidieri, Mariel Silvina
Conci, Vilma Cecilia
Resumen
A new potyvirus, tentatively named cucurbit vein banding virus (CVBV), was identified in crops of cucurbits in San Pedro (Buenos Aires, Argentina). The complete genome sequences of two isolates of CVBV were obtained by next-generation sequencing (Illumina). The genomic RNA consisted of 9968 and 9813 nucleotides, respectively, and displayed typical potyvirus organization. The percentage identity for these two genome sequences, using BLASTn, was 77% to sweet potato virus c and 73% to tomato necrotic stunt virus. BLASTx analysis of the complete polyprotein showed that the most closely related virus is plum pox virus, with 48% amino acid sequence identity for both isolates. Sequence comparisons and phylogenetic analyses indicate that CVBV belongs to a previously undescribed species in genus Potyvirus.