Fabrication of a novel biosensor for biosensing of bisphenol A and detection of its damage to DNA

Abstract

In this work, a novel electrochemical biosensor has been fabricated based on step-by-step modification of aglassy carbon electrode (GCE) with methylene blue (MB)-DNA/multiwalled carbon nanotubes (MWCNTs)-chitosan (CS)/palladium nanoparticles (Pd NPs)/fullerene C60 (C60) for voltammetric and impedimetric detection of DNA damage induced by bisphenol A (BPA). Modifications applied to the GCE were characterized bycyclic voltammetry (CV), differential pulse voltammetry (DPV), electrochemical impedance spectroscopy (EIS)and scanning electron microscopy. The EIS and DPV responses of the biosensor were increased and decreased,respectively, by the DNA damage induced by BPA which led us to develop novel systems for detection of DNAdamage. Our records confirmed that the biosensor was able to rapid and sensitive detection of DNA damageinduced by BPA. Finally, according to the developed systems for detection of DNA damage, we have developedvoltammetric and impedimetric methods for determination of BPA