info:eu-repo/semantics/article
Anti HSV-1 activity of halistanol sulfate and halistanol sulfate C isolated from Brazilian marine sponge Petromica citrina (Demospongiae)
Fecha
2013-11Registro en:
Guimarães, Tatiana Da Rosa; Quiroz, Carlos Guillermo; Rigotto, Caroline; De Oliveira, Simone Quintana; Rojo de Almeida, Maria Tereza; et al.; Anti HSV-1 activity of halistanol sulfate and halistanol sulfate C isolated from Brazilian marine sponge Petromica citrina (Demospongiae); Molecular Diversity Preservation International; Marine Drugs; 11; 11; 11-2013; 4176-4192
1660-3397
CONICET Digital
CONICET
Autor
Guimarães, Tatiana Da Rosa
Quiroz, Carlos Guillermo
Rigotto, Caroline
De Oliveira, Simone Quintana
Rojo de Almeida, Maria Tereza
Bianco, Éverson Miguel
Moritz, Maria Izabel Goulart
Carraro, João Luís
Palermo, Jorge Alejandro
Cabrera, Gabriela Myriam
Schenkel, Eloir Paulo
Reginatto, Flávio Henrique
Oliveira Simões, Cláudia Maria
Resumen
The n-butanol fraction (BF) obtained from the crude extract of the marine sponge Petromica citrina, the halistanol-enriched fraction (TSH fraction), and the isolated compounds halistanol sulfate (1) and halistanol sulfate C (2), were evaluated for their inhibitory effects on the replication of the Herpes Simplex Virus type 1 (HSV-1, KOS strain) by the viral plaque number reduction assay. The TSH fraction was the most effective against HSV-1 replication (SI = 15.33), whereas compounds 1 (SI = 2.46) and 2 (SI = 1.95) were less active. The most active fraction and these compounds were also assayed to determine the viral multiplication step(s) upon which they act as well as their potential synergistic effects. The anti-HSV-1 activity detected was mediated by the inhibition of virus attachment and by the penetration into Vero cells, the virucidal effect on virus particles, and by the impairment in levels of ICP27 and gD proteins of HSV-1. In summary, these results suggest that the anti-HSV-1 activity of TSH fraction detected is possibly related to the synergic effects of compounds 1 and 2.