info:eu-repo/semantics/article
Effect of nano-sized bioactive glass particles on the angiogenic properties of collagen based composites
Fecha
2013-02-22Registro en:
Vargas, Gabriela Elizabet; Haro Durand, Luis Alberto; Cadena, María Vanesa; Romero, Marcela; Vera Mesones, Rosa; et al.; Effect of nano-sized bioactive glass particles on the angiogenic properties of collagen based composites; Springer; Journal of Materials Science: Materials In Medicine; 24; 5; 22-2-2013; 1261-1269
0957-4530
CONICET Digital
CONICET
Autor
Vargas, Gabriela Elizabet
Haro Durand, Luis Alberto
Cadena, María Vanesa
Romero, Marcela
Vera Mesones, Rosa
Mačković, Mirza
Spallek, Stefanie
Spiecker, Erdmann
Boccaccini, Aldo R.
Gorustovich Alonso, Alejandro Adrian
Resumen
Angiogenesis is essential for tissue regeneration and repair. A growing body of evidence shows that the use of bioactive glasses (BG) in biomaterial-based tissue engineering (TE) strategies may improve angiogenesis and induce increased vascularization in TE constructs. This work investigated the effect of adding nano-sized BG particles (n-BG) on the angiogenic properties of bovine type I collagen/n-BG composites. Nano-sized (20-30 nm) BG particles of nominally 45S5 Bioglass composition were used to prepare composite films, which were characterized by scanning electron microscopy (SEM) and transmission electron microscopy (TEM). The in vivo angiogenic response was evaluated using the quail chorioallantoic membrane (CAM) as an model of angiogenesis. At 24 h post-implantation, 10 wt% n-BG containing collagen films stimulated angiogenesis by increasing by 41 % the number of blood vessels branch points. In contrast, composite films containing 20 wt% n-BG were found to inhibit angiogenesis. This experimental study provides the first evidence that addition of a limited concentration of n-BG (10 wt%) to collagen films induces an early angiogenic response making selected collagen/n-BG composites attractive matrices for tissue engineering and regenerative medicine.