info:eu-repo/semantics/article
Anthelmintic activity of Stevia multiaristata extract against Echinococcus granulosus sensu stricto
Fecha
2021-12Registro en:
Albani, Clara Maria; Borgo, Jimena; Fabbri, Julia; Pensel, Patricia Eugenia; Fasciani, Lara; et al.; Anthelmintic activity of Stevia multiaristata extract against Echinococcus granulosus sensu stricto; Cambridge University Press; Parasitology; 149; 4; 12-2021; 519-528
0031-1820
1469-8161
CONICET Digital
CONICET
Autor
Albani, Clara Maria
Borgo, Jimena
Fabbri, Julia
Pensel, Patricia Eugenia
Fasciani, Lara
Elso, Orlando German
Papademetrio, Daniela Laura
Grasso, Daniel Hector
Paladini, Antonela
Beer, Maria Florencia
Farias, Nahuel Emiliano
Elissondo, Natalia
Gambino, Guillermo
Zoppi, Jorge
Sülsen, Valeria Patricia
Elissondo, María Celina
Resumen
Cystic echinococcosis is a zoonotic disease caused by the larval stage of the parasite Echinococcus granulosus sensu lato. The available anti-parasitic treatment is mostly limited to a continuous administration of albendazole. However, due to its numerous side-effects and efficacy of around 50%, there is a need to find new drugs to improve the treatment for this disease. In the current study, the in vitro and in vivo efficacy of a Stevia multiaristata extract against E. granulosus sensu stricto (s.s.) was demonstrated. Stevia multiaristata extract (100 and 50 g mL-1) caused a quick viability decrease on protoscoleces which was consistent with the observed tegumental alterations. Loss of turgidity was detected in 95 ± 3.4% of cysts incubated with S. multiaristata extract during 2 days (100 g mL-1) and the collapse of the germinal layer was observed in 60 ± 9.3% of cysts treated with 100 g mL-1 of the S. multiaristata extract during 4 days. The half maximal effective concentration value was 69.6 g mL-1 and the selectivity index for E. granulosus s.s. cysts was 1.9. In this clinical efficacy study, the treatment of infected mice with the S. multiaristata extract (50 mg kg-1) caused a significant decrease in the weight of the cysts compared with the control group. These results coincided with the tissue damage observed in the cysts at the ultrastructural level. In conclusion, we observed high protoscolicidal and cysticidal effects, and significant reduction in the weight of the cysts in experimentally infected mice following treatment with the S. multiaristata extract.