info:eu-repo/semantics/article
Increasing the digital repository of DNA barcoding sequences of sand flies (Psychodidae: Phlebotominae)
Fecha
2019-11-28Registro en:
Laurito, Magdalena; Ontivero, Iliana Mayra; Almiron, Walter Ricardo; Increasing the digital repository of DNA barcoding sequences of sand flies (Psychodidae: Phlebotominae); Fundação Oswaldo Cruz; Memórias do Instituto Oswaldo Cruz; 114; 11; 28-11-2019
0074-0276
1678-8060
CONICET Digital
CONICET
Autor
Laurito, Magdalena
Ontivero, Iliana Mayra
Almiron, Walter Ricardo
Resumen
Sand f ly identification is complex because it depends on the expertise of the taxonomist. The females show subtle morphological differences and the occurrence of the species complexes are usual in this taxon. Therefore, a fragment of the cytochrome c oxidase subunit I (COI) gene is used for taxon barcoding to resolve this kind of problem. This study incorporates barcode sequences, for the first time, for Evandromyia cortelezzii and Migonemyia migonei from Argentina. The nucleotide sequence divergences were estimated to generate a neighbour-joining (NJ) tree. The automatic barcode gap discovery (ABGD) approach was employed to find the barcode gaps and the operational taxonomic unit (OTU) delimitation. Other species of the subtribe were included. The frequency histogram of divergences showed a barcoding gap. The ABGD analysis identified 14 operational taxonomic units (OTUs) from 13 morphological species. Sequences of Ev. cortelezzii and Mg. migonei formed well supported clusters and were diagnosed as primary species. These sequences are useful tools for molecular identification of the sand f lies of the New World.