info:eu-repo/semantics/article
Single step recombinant human growth hormone (rhGH) purification from milk by peptide affinity chromatography
Fecha
2018-07Registro en:
Saavedra, Soledad Lorena; Martínez Ceron, María Camila; Giudicessi, Silvana Laura; Forno, Angela Guillermina; Bosco, Maria Belen; et al.; Single step recombinant human growth hormone (rhGH) purification from milk by peptide affinity chromatography; American Chemical Society; Biotechnology Progress; 34; 4; 7-2018; 999-1005
8756-7938
CONICET Digital
CONICET
Autor
Saavedra, Soledad Lorena
Martínez Ceron, María Camila
Giudicessi, Silvana Laura
Forno, Angela Guillermina
Bosco, Maria Belen
Marani, Mariela Mirta
Erra Balsells, Rosa
Albericio Palomera, Fernando
Cascone, Osvaldo
Camperi, Silvia Andrea
Resumen
Recombinant human growth hormone (rhGH) is used for the treatment of several pathologies, most of them related to growth. Although different expression systems can be used for its production, the milk from transgenic cows is one of the most interesting due to the high rhGH level achieved (5 g/L). We have designed and synthesized short peptides (9 or 10 amino acid long) using Fmoc chemistry and studied their ability to purify rhGH from milk once immobilized on an agarose support. Using spiked milk with the hormone as a sample, rhGH was purified with 88% yield and 92% purity in a single step with a fold purification of 4.5.