info:eu-repo/semantics/article
Urinary vanillylmandelic acid:creatinine ratio in dogs with pheochromocytoma
Fecha
2021-01Registro en:
Soler Arias, Elber Alberto; Trigo, Roberto Héctor; Miceli, Diego Daniel; Vidal, Patricia Noemi; Hernandez Blanco, M. F.; et al.; Urinary vanillylmandelic acid:creatinine ratio in dogs with pheochromocytoma; Elsevier Science Inc.; Domestic Animal Endocrinology; 74; 106559; 1-2021; 1-7
0739-7240
CONICET Digital
CONICET
Autor
Soler Arias, Elber Alberto
Trigo, Roberto Héctor
Miceli, Diego Daniel
Vidal, Patricia Noemi
Hernandez Blanco, M. F.
Castillo, Victor Alejandro
Resumen
Pheochromocytoma diagnosis in dogs is challenging because biochemical tests are not always available. In humans, urinary vanillylmandelic acid (VMA) is part of a pheochromocytoma biochemical diagnostic profile, whereas its diagnostic accuracy is currently unknown in dogs with pheochromocytoma. Prospectively, VMA was determined by HPLC and expressed as the ratio with respect to urinary creatinine (VMA:C). The diagnostic accuracy of the VMA:C ratio was evaluated by analyzing the receiver operating characteristic (ROC) curve in 10 healthy dogs, 8 dogs with pituitary-dependent hypercortisolism, 8 dogs with adrenal-dependent hypercortisolism, and 7 dogs with pheochromocytoma. The pheochromocytoma diagnosis was confirmed by histology and immunohistochemistry in all tumors. The VMA:C ratio was significantly higher in dogs with pheochromocytoma (158 [53.4 to 230.8] × 10−3) than in dogs with adrenal-dependent hypercortisolism (48.1 [24.3 to 144.9] × 10−3; P < 0.05), dogs with pituitary-dependent hypercortisolism (37.5 [32 to 47.1] × 10−3; P < 0.001), and healthy dogs (33.8 [13.3 to 87.9] × 10−3; P < 0.001). When using a VMA:C ratio >58.2 × 10−3 for pheochromocytoma diagnosis, a sensitivity of 85.7% and a specificity of 88.4% were obtained. Nevertheless, when using a cut-off ratio of 4 times the median VMA:C ratio determined in healthy dogs, there was no overlap (100% specificity). The area under the ROC curve indicated that the VMA:C ratio test could be used to discriminate between dogs with and without pheochromocytoma, what leads to the conclusion that it is useful for pheochromocytoma diagnosis in dogs.