info:eu-repo/semantics/article
A combination of sbmA and tolC mutations in Escherichia coli K-12 Tn10-carrying strains results in hypersusceptibility to tetracycline
Fecha
2008-02Registro en:
de Cristobal, Ricardo Ezequiel; Vincent, Paula Andrea; Salomon, Raul Armando; A combination of sbmA and tolC mutations in Escherichia coli K-12 Tn10-carrying strains results in hypersusceptibility to tetracycline; American Society for Microbiology; Journal of Bacteriology; 190; 4; 2-2008; 1491-1494
0021-9193
CONICET Digital
CONICET
Autor
de Cristobal, Ricardo Ezequiel
Vincent, Paula Andrea
Salomon, Raul Armando
Resumen
Previously, we demonstrated that Escherichia coli tolC mutations reduce the high-level resistance to tetracycline afforded by the transposon Tn10-encoded TetA pump from resistance at 200 μg/ml to resistance at 40 μg/ml. In this study, we found that the addition of an sbmA mutation to a tolC::Tn10 mutant exacerbates this phenotype: the double mutant did not form colonies, even in the presence of tetracycline at a concentration as low as 5 μg/ml. Inactivation of sbmA alone partially inhibited high-level tetracycline resistance, from resistance at 200 μg/ml to resistance at 120 μg/ml. There thus appears to be an additive effect of the mutations, resulting in almost complete suppression of the phenotypic expression of Tn10 tetracycline resistance. Copyright © 2008, American Society for Microbiology. All Rights Reserved.