info:eu-repo/semantics/article
Increased leukotriene concentration in submandibular glands from rats with experimental periodontitis
Date
2009-04Registration in:
Busch, Lucila; Miozza, Valeria Alejandra; Sterin, Leonor Josefina; Borda, Enri Santiago; Increased leukotriene concentration in submandibular glands from rats with experimental periodontitis; Springer; Inflammation Research; 58; 7; 4-2009; 423-430
1023-3830
CONICET Digital
CONICET
Author
Busch, Lucila
Miozza, Valeria Alejandra
Sterin, Leonor Josefina
Borda, Enri Santiago
Abstract
Objective and design: In the present study, we investigated the relation between the inflammatory mediators such as nitric oxide, prostaglandins, and cysteinyl-leukotrienes with mucin release and the sympathetic system in submandibular glands from rats with experimental periodontitis. Materials or subjects: Submandibular glands from rats with experimental periodontitis. Treatment: For the first experiment, rats were treated with hydrocortisone sc, 1 mg/kg for 3 days. All other experiments were carried out in isolated submandibular glands from untreated rats. Submandibular glands were treated with cysteinyl-leukotrienes, isoproterenol, NDGA, FPL 55712, L-NMMA, Nio, Nz, AMG, indomethacin, DuP 697 and atenolol. Methods: Nitric oxide synthase activity, prostaglandin and cysteinyl-leukotriene productions and mucin secretion were determined. The Newman-Keuls statistical test was applied after analysis of variance. Results: In rats with periodontitis hydrocortisone-induced a 36.6% (P < 0.05) decrease in mucin release. Only cysteinyl-leukotriene production was increased in rats with ligature (79.2%, P < 0.001). Either the inhibition of cysteinyl-leukotriene production or the block of leukotriene receptor abolished the increase in mucin secretion by 25.6% (P < 0.05) and 37% (P < 0.01), respectively, in glands from rats with ligature. On the other hand, the presence of cysteinyl-leukotrienes in the incubation medium induced mucin release from submandibular glands. Atenolol diminished by 24% (P < 0.05), the increase in cysteinyl-leukotrienes observed in rats with periodontitis. Besides, isoproterenol induced cysteinyl-leukotriene production in both groups. Conclusion: In submandibular glands from rats with periodontitis, the increment in mucin release and cysteinyl-leukotrienes production are related events and both are associated with the sympathetic system.