info:eu-repo/semantics/article
An improved staining protocol for the assessment of arbuscular mycorrhizal in bryophytes
Fecha
2018-07Registro en:
Cottet, Agustina Celeste; Scervino, Jose Martin; Messuti, Maria Ines; An improved staining protocol for the assessment of arbuscular mycorrhizal in bryophytes; Sociedad Argentina de Botánica; Boletín de la Sociedad Argentina de Botánica; 53; 2; 7-2018; 201-206
0373-580X
1851-2372
CONICET Digital
CONICET
Autor
Cottet, Agustina Celeste
Scervino, Jose Martin
Messuti, Maria Ines
Resumen
The most accepted method for staining arbuscular mycorrhiza (AM) in vascular plants has been one proposed by Phillips & Hayman in 1970. In particular, for the study of AM in bryophytes (s.l.) [Anthocerotophyta, Bryophyta (s.s.), Marchantiophyta] some authors have introduced modifications tothis technique. Even though all these protocols stain AM, their main disadvantage is related to the result of material maceration (e.g. over-softening or completely destroying plant cells due to the high temperaturesused, the high concentrations of reagents or the long-term exposure to aggressive chemicals). In order to optimise the results for the observation of AM in this group of plants, a modification is presented to the traditional staining technique. In the protocol here proposed, 70% ethanol is used as fixative and first clarifier, 1% potassium hydroxide (KOH) (80 °C, 20 min) as a second clarifier; 1% hydrochloric acid (HCl) (50 °C, 10 min) as an acidifier and 0.05% trypan blue (60 °C, 20 min) for dyeing. This improved protocol is not destructive, it is fast to perform and it is of wide application since it allows staining the AM in bryophytes.