info:eu-repo/semantics/article
PAH removal by simultaneous and sequential inoculation of Pseudomonas monteilii P26 and Gordonia sp. H19 in the presence of biostimulants
Date
2019-10Registration in:
Juárez Tomás, María Silvina; Carrasco, Marina Gabriela; Lobo, Constanza Belén; Alessandrello, Mauricio Javier; Sanchez, Leandro Arturo; et al.; PAH removal by simultaneous and sequential inoculation of Pseudomonas monteilii P26 and Gordonia sp. H19 in the presence of biostimulants; Elsevier; International Biodeterioration and Biodegradation; 144; 10-2019
0964-8305
CONICET Digital
CONICET
Author
Juárez Tomás, María Silvina
Carrasco, Marina Gabriela
Lobo, Constanza Belén
Alessandrello, Mauricio Javier
Sanchez, Leandro Arturo
Ferrero, Marcela Alejandra
Abstract
Biostimulation and bioaugmentation have been proposed as sustainable alternatives to physicochemical treatments for the environmental decontamination of polycyclic aromatic hydrocarbons (PAH). PAH-removing Pseudomonas monteilii P26 and Gordonia sp. H19, alone or combined with organic and inorganic biostimulants, were previously proposed as candidates to be included in biotechnological products to be used in eco-friendly decontamination processes. This work aims to study the effect of biostimulants on the growth, cell hydrophobicity and emulsifying activity of P. monteilii P26 and Gordonia sp. H19, and to evaluate the influence of selected biostimulants and bacterial inoculation procedure on PAH bioremoval. A complete factorial experimental design was applied to determine the effect of KNO3, K2HPO4, corn steep liquor (CSL) and a PAH mixture on the growth, cell hydrophobicity and bioemulsifying activity of bacterial cultures. The kinetics of PAH bioremoval was evaluated in simultaneous and sequential cultures of P. monteilii P26 and Gordonia sp. H19 in the presence of selected biostimulants. CSL was the only nutrient that exerted positive effects on all responses evaluated, although these effects were not significant in all cases. In mixed cultures, maximum bioremoval of phenanthrene and pyrene was evidenced when the bacterial strains were simultaneously inoculated, independently of the presence or absence of CSL. These results support the biotechnological characterization of two PAH-removing autochthonous Patagonian strains.