info:eu-repo/semantics/article
Optimization of bromelain isolation from pineapple byproducts by polysaccharide complex formation
Fecha
2019-02Registro en:
Campos, Débora; Coscueta, Ezequiel Ricardo; Woitovich Valetti, Nadia; Pastrana Castro, Lorenzo M.; Teixeira, José; et al.; Optimization of bromelain isolation from pineapple byproducts by polysaccharide complex formation; Elsevier; Food Hydrocolloids; 87; 2-2019; 792-804
0268-005X
CONICET Digital
CONICET
Autor
Campos, Débora
Coscueta, Ezequiel Ricardo
Woitovich Valetti, Nadia
Pastrana Castro, Lorenzo M.
Teixeira, José
Picó, Guillermo Alfredo
Pintado, Maria Manuela
Resumen
A simple method for bromelain extraction from industrial pineapple residues (stems and peels) was developed and optimized through factorial experimental design. The developed methodology, based on precipitation with carrageenan, represents an alternative to the use of organic solvents and inorganic salts (common industrial precipitation) and allows achieving extracts with high bromelain purity. High recovery yield – 80–90% - of active bromelain was obtained for both crude juices (stems and peels) making possible to obtain ca. 0.3 g of bromelain from 100 g of pineapple byproducts using a low polysaccharide concentration (0.2–0.3% w/v).