info:eu-repo/semantics/article
High-frequency neuronal bursting is essential for circadian and sleep behaviors in drosophila
Fecha
2021-01Registro en:
Fernández, Florencia; Frenkel, Lia; Colque, Carina Celeste; Ricciuti, Ana; Hahm, Bryan; et al.; High-frequency neuronal bursting is essential for circadian and sleep behaviors in drosophila; Society for Neuroscience; Journal of Neuroscience; 41; 4; 1-2021; 689-710
0270-6474
CONICET Digital
CONICET
Autor
Fernández, Florencia
Frenkel, Lia
Colque, Carina Celeste
Ricciuti, Ana
Hahm, Bryan
Cerredo, Karina
Muraro, Nara Ines
Ceriani, Maria Fernanda
Resumen
Circadian rhythms have been extensively studied in Drosophila; however, still little is known about how the electrical properties of clock neurons are specified. We have performed a behavioral genetic screen through the downregulation of candidate ion channels in the lateral ventral neurons (LNvs) and show that the hyperpolarization-activated cation current Ih is important for the behaviors that the LNvs influence: temporal organization of locomotor activity, analyzed in males, and sleep, analyzed in females. Using whole-cell patch clamp electrophysiology we demonstrate that small LNvs (sLNvs) are bursting neurons, and that Ih is necessary to achieve the high-frequency bursting firing pattern characteristic of both types of LNvs in females. Since firing in bursts has been associated to neuropeptide release, we hypothesized that Ih would be important for LNvs communication. Indeed, herein we demonstrate that Ih is fundamental for the recruitment of pigment dispersing factor (PDF) filled dense core vesicles (DCVs) to the terminals at the dorsal protocerebrum and for their timed release, and hence for the temporal coordination of circadian behaviors.