info:eu-repo/semantics/article
In vitro methods to study the modulation of migration and invasion by sphingosine-1-phosphate
Fecha
2017-04Registro en:
Castro, Melina Gabriela; Campos, Ludmila Estefanía; Rodriguez, Yamila Isabel; Alvarez, Sergio Eduardo; In vitro methods to study the modulation of migration and invasion by sphingosine-1-phosphate; Humana Press; Methods in Molecular Biology; 1697; 4-2017; 117-131
978-1-4939-7413-9
1064-3745
CONICET Digital
CONICET
Autor
Castro, Melina Gabriela
Campos, Ludmila Estefanía
Rodriguez, Yamila Isabel
Alvarez, Sergio Eduardo
Resumen
Sphingosine-1-phosphate (S1P) is a bioactive lipid that modulates migratory behavior of cells during embryonic development. In addition, S1P might promote tumor progression by enhancing migratory ability and invasiveness of tumor cells. Migration is a complex process that implies cytoskeletal reorganization and formation of structures that enable cell movement. Besides having similar requirements than migration, invasion also involves proteolytic degradation of extracellular matrix (ECM). Matrix metalloproteases (MMPs) have been identified to break down components of the ECM, allowing cancer cells to spread out of the primary tumor. In this chapter, we will describe different techniques to study migration and invasion induced by S1P. To this end, we include detailed protocols of end-point assays to study migration/invasion, and zymography assay to analyze MMP-2 and MMP-9 activity that were standardized in our laboratory in human melanoma cell lines.