info:eu-repo/semantics/article
Protein kinase D1 inhibition interferes with mitosis progression
Fecha
2019-03-25Registro en:
Martínez León, Eduardo Antonio; Amable, Gastón Federico; Jácamo, Rodrigo; Picco, María Elisa; Anaya, Laura; et al.; Protein kinase D1 inhibition interferes with mitosis progression; Wiley-liss, Div John Wiley & Sons Inc; Journal of Cellular Physiology; 234; 11; 25-3-2019; 20510-20519
0021-9541
CONICET Digital
CONICET
Autor
Martínez León, Eduardo Antonio
Amable, Gastón Federico
Jácamo, Rodrigo
Picco, María Elisa
Anaya, Laura
Rozengurt, Enrique
Rey, Osvaldo
Resumen
Protein kinase D1 (PKD1) plays a vital role in signal transduction, cell proliferation, membrane trafficking, and cancer; however, the majority of the studies up to date had centered primarily on PKD1 functions in interphase, very little is known about its role during cell division. We previously demonstrated that during mitosis PKD1 is activated and associated with centrosomes, spindles, and midbodies. However, these observations did not address whether PKD1 was associated with mitosis regulation. Accordingly, we used rapidly acting PKD‐specific inhibitors to examine the contribution of PKD1 the sequence of events in mitosis. We found that although PKD1 overexpression did not affect mitosis progression, suppression of its catalytic activity by two structurally unrelated inhibitors (kb NB 142‐70 and CRT 0066101) induced a significant delay in metaphase to anaphase transition time. PKD1 inhibition during mitosis also produced the appearance of abnormal spindles, defects in chromosome alignment, and segregation as well as apoptosis. Thus, these observations indicate that PKD1 activity is associated with mitosis regulation.