info:eu-repo/semantics/article
Chiral separation of several pesticides on an immobilized amylose tris(3-chloro-5-methylphenylcarbamate) column under polar-organic conditions: influence of mobile phase and temperature on enantioselectivity
Fecha
2020-08Registro en:
Diaz Merino, Matias Ezequiel; Lancioni, Carlina; Padró, Juan Manuel; Castells, Cecilia Beatriz Marta; Chiral separation of several pesticides on an immobilized amylose tris(3-chloro-5-methylphenylcarbamate) column under polar-organic conditions: influence of mobile phase and temperature on enantioselectivity; Elsevier; Journal of Chromatography - A; 1624; 8-2020; 1-9
0021-9673
CONICET Digital
CONICET
Autor
Diaz Merino, Matias Ezequiel
Lancioni, Carlina
Padró, Juan Manuel
Castells, Cecilia Beatriz Marta
Resumen
In this work, the use of different solvents and temperatures was explored, aiming to evaluate their influence on the enantioseparation of pesticides by HPLC in polar-organic conditions, employing a column containing immobilized amylose tris(3-chloro-5-methylphenyl-carbamate). The chiral separation of seventeen different pesticides widely used as herbicides, fungicides, insecticides and precursors were studied. The mobile phases included methanol, ethanol, iso-propanol, n-propanol and acetonitrile; either pure or containing additives such as diethylamine, trifluoroacetic acid, formic acid, acetic acid or mixtures thereof. We studied the influence of these eluents on chiral separation of those pesticides in terms of retention factor, enantioselectivity, enantioresolution and peak symmetry. Regarding temperature influence, evaluated within the range 5 – 40 °C, nearly all the compounds decreased their retention and selectivity factors with the increase in temperature, although the effect was dependent on the mobile phase solvent. Moreover, estimation of thermodynamic parameters was performed based on linear van´t Hoff plots.