info:eu-repo/semantics/article
Slurry sampling in serum blood for mercury determination by CV-AFS
Fecha
2009-01Registro en:
Aranda, Pedro Rodolfo; Gil, Raul Andres; Moyano, Susana; de Vito, Irma Esther; Martinez, Luis Dante; Slurry sampling in serum blood for mercury determination by CV-AFS; Elsevier Science; Journal of Hazardous Materials; 161; 2-3; 1-2009; 1399-1403
0304-3894
CONICET Digital
CONICET
Autor
Aranda, Pedro Rodolfo
Gil, Raul Andres
Moyano, Susana
de Vito, Irma Esther
Martinez, Luis Dante
Resumen
The heavy metal mercury (Hg) is a neurotoxin known to have a serious health impact even at relatively low concentrations. A slurry method was developed for the sensitive and precise determination of mercury in human serum blood samples by cold vapor generation coupled to atomic fluorescence spectrometry (CV-AFS). All variables related to the slurry formation were studied. The optimal hydrochloric concentration and tin(II) chloride concentration for CV generation were evaluated. Calibration within the range 0.1-10 μg L-1 Hg was performed with the standard addition method, and compared with an external calibration. Additionally, the reliability of the results obtained was evaluated by analyzing mercury in the same samples, but submitted to microwave-assisted digestion method. The limit of detection was calculated as 25 ng L-1 and the relative standard deviation was 3.9% at levels around of 0.4 μg L-1 Hg.