info:eu-repo/semantics/article
A kinetic approach to assess oxidative metabolism related features in the bivalve Mya arenaria
Fecha
2012-12Registro en:
González, Paula Mariela; Abele, Doris; Puntarulo, Susana Ángela; A kinetic approach to assess oxidative metabolism related features in the bivalve Mya arenaria; Springer; Theory In Biosciences; 131; 4; 12-2012; 253-264
1431-7613
1611-7530
CONICET Digital
CONICET
Autor
González, Paula Mariela
Abele, Doris
Puntarulo, Susana Ángela
Resumen
Electron paramagnetic resonance uses the resonant microwave radiation absorption of paramagnetic substances to detect highly reactive and, therefore, shortlived oxygen and nitrogen centered radicals. Previously, steady state concentrations of nitric oxide, ascorbyl radical (A·) and the labile iron pool (LIP) were determined in digestive gland of freshly collected animals from the North Sea bivalve Mya arenaria. The application of a simple kinetic analysis of these data based on elemental reactions allowed us to estimate the steady state concentrations of superoxide anion, the rate of A· disappearance and the content of unsaturated lipids. This analysis applied to a marine invertebrate opens the possibility of a mechanistic understanding of the complexity of free radical and LIP interactions in a metabolically slow, cold water organism under unstressed conditions. This data can be further used as a basis to assess the cellular response to stress in a simple system as the bivalve M. arenaria that can then be compared to cells of higher organisms.