info:eu-repo/semantics/article
Chronic exposure to low-dose of X-rays induces DNA double-strand breaks in mammalian cells
Fecha
2011-10Registro en:
Grillo, Claudia Alejandra; Güerci, Alba Mabel; Chronic exposure to low-dose of X-rays induces DNA double-strand breaks in mammalian cells; Inderscience Enterprises; International Journal of Low Radiation; 8; 3; 10-2011; 198-213
1477-6545
1741-9190
CONICET Digital
CONICET
Autor
Grillo, Claudia Alejandra
Güerci, Alba Mabel
Resumen
The purpose of present study is to evaluate the induction of clastogenic effect after chronic exposure to low dose of ionising radiation in both CHO-K1 cells and in their DNA Double-Strand Breaks (DSBs)-repair deficient mutant xrs-5. Cells were cultured for 14 dilution cycles and grown to confluency. Radiation treatment was performed once per cycle with 10 mGy X-rays to carry out the structural chromosome aberration test. The results showed a significant increase in the frequency of achromatic lesions (p < 0.001) in CHO-K1 cells. In addition, xrs-5 cells showed an increase of chromatid and chromosome-type aberrations (p < 0.001). Sequential exposure produced a level of DNA damage that is amplified through successive cycles triggering the efficient repair of DSBs. The increased damage in xrs-5 cells provides evidence that the non-homologous end-joining repair of DNA DSBs is involved after low dose X-ray exposure.