info:eu-repo/semantics/article
Membrane localization of Junín virus glycoproteins requires cholesterol and cholesterol rich membranes
Fecha
2013-01Registro en:
Cordo, Sandra Myriam; Valko, Ayelén; Martinez, María Guadalupe; Candurra, Nélida Alicia; Membrane localization of Junín virus glycoproteins requires cholesterol and cholesterol rich membranes; Academic Press Inc Elsevier Science; Biochemical and Biophysical Research Communications; 430; 3; 1-2013; 912-917
0006-291X
CONICET Digital
CONICET
Autor
Cordo, Sandra Myriam
Valko, Ayelén
Martinez, María Guadalupe
Candurra, Nélida Alicia
Resumen
Arenavirus morphogenesis and budding occurs at cellular plasma membrane;however, the nature of membrane assembly sites remains poorly understood. In this study we examined the effect of different cholesterol-lowering agents on Junín virus (JUNV) multiplication. We found that cholesterol cell depletion reduced JUNV glycoproteins (GPs) membrane expression and virus budding. Analysis of membrane protein insolubility in Triton X-100 suggested that JUNV GPs associate with cholesterol enriched membranes. Rafts dissociation conditions as warm detergent extraction and cholesterol removal by methyl-b-cyclodextrin compound showed to impair GPs cholesterol enriched membrane association.Analysis of GPs transfected cells showed similar results suggesting that membrane raft association is independent of other viral proteins.