info:eu-repo/semantics/article
Urban air pollution induces redox imbalance and epithelium hyperplasia in mice cornea
Fecha
2019-12-01Registro en:
Lasagni Vitar, Romina Mayra; Hvozda Arana, Ailen Gala; Janezic, Natasha S.; Marchini, Timoteo Oscar; Tau, Julia; et al.; Urban air pollution induces redox imbalance and epithelium hyperplasia in mice cornea; Academic Press Inc Elsevier Science; Toxicology and Applied Pharmacology; 384; 114770; 1-12-2019; 1-37
0041-008X
CONICET Digital
CONICET
Autor
Lasagni Vitar, Romina Mayra
Hvozda Arana, Ailen Gala
Janezic, Natasha S.
Marchini, Timoteo Oscar
Tau, Julia
Martinefski, Manuela Romina
Tesone, Agustina Inés
Racca, Lourdes
Reides, Claudia Gabriela
Tripodi, Valeria Paula
Evelson, Pablo Andrés
Berra, Alejandro
Llesuy, Susana Francisca
Ferreira, Sandra María
Resumen
The aim of the study was to evaluate the time course of the effects of urban air pollutants on the ocular surface, focusing on the morphological changes, the redox balance, and the inflammatory response of the cornea. 8-week-old mice were exposed to urban or filtered air (UA-group and FA-group, respectively) in exposure chambers for 1, 2, 4, and 12 weeks. After each time, the eyes were enucleated and the corneas were isolated for biochemical analysis. UA-group corneas exhibited a continuous increase in NADPH oxidase-4 levels throughout the exposure time, suggesting an increased production of reactive oxygen species (ROS). After 1 week, an early adaptive response to ROS was observed as an increase in antioxidant enzymes. After 4 weeks, the enzymatic antioxidants were decreased, meanwhile an increase of the glutathione was shown, as a later compensatory antioxidant response. However, redox imbalance took place, evidenced by the increased oxidized proteins, which persisted up to 12 weeks. At this time point, corneal epithelium hyperplasia was also observed. The inflammatory response was modulated by the increase in IL-10 levels after 1 week, which early regulates the release of TNF-α and IL-6. These results suggest that air pollution alters the ocular surface, supported by the observed cellular hyperplasia. The redox imbalance and the inflammatory response modulated by IL-10 play a key role in the response triggered by air pollutants on the cornea. Taking into account this time course study, the ocular surface should also be considered as a relevant target of urban air pollutants.