info:eu-repo/semantics/article
Compatibility and safety of five lectin-binding putative probiotic strains for the development of a multi-strain protective culture for poultry
Fecha
2018-08Registro en:
Babot, Jaime Daniel; Argañaraz Martínez, Fernando Eloy; Saavedra, Maria Lucila; Apella, Maria Cristina; Perez Chaia, Adriana Beatriz; Compatibility and safety of five lectin-binding putative probiotic strains for the development of a multi-strain protective culture for poultry; Wageningen Academic Publishers; Beneficial Microbes; 9; 6; 8-2018; 927-935
1876-2883
1876-2891
CONICET Digital
CONICET
Autor
Babot, Jaime Daniel
Argañaraz Martínez, Fernando Eloy
Saavedra, Maria Lucila
Apella, Maria Cristina
Perez Chaia, Adriana Beatriz
Resumen
The ban on the use of antibiotics as feed additives for animal growth promotion in the European Union and United States and the expectation of this trend to further expand to other countries in the short term have prompted a surge in probiotic research. Multi-species probiotics including safe and compatible strains with the ability to bind different nutritional lectins with detrimental effects on poultry nutrition could replace antibiotics as feed additives. Lactobacillus salivarius LET201, Lactobacillus reuteri LET210, Enterococcus faecium LET301, Propionibacterium acidipropionici LET103 and Bifidobacterium infantis CRL1395 have proved to be compatible as evaluated through three different approaches: the production and excretion of antimicrobial compounds, growth inhibition by competition for essential nutrients and physical contact, and a combination of both. The safety of P. acidipropionici LET103 was confirmed, since no expression of virulence factors or antibiotic resistance was detected. The innocuity of E. faecium LET301 should be further evaluated, since the presence of genes coding for certain virulence factors (gelE, efaAfm and efaAfs) was observed, albeit no expression of gelE was previously detected for this strain and there are no reports of involvement of efaAfm in animal pathogenicity. Finally, a combination of the five strains effectively protected intestinal epithelial cells of broilers from the cytotoxicity of mixtures of soybean agglutinin, wheat germ agglutinin and concanavalin A. To our knowledge, this is the first time that a combination of strains is evaluated for their protection against lectins that might be simultaneously present in poultry feeds.