info:eu-repo/semantics/article
Epicatechin protects thoracic aortic perivascular adipose tissue from whitening in high-fat fed mice
Fecha
2020-07Registro en:
Hid, Ezequiel José; Fischerman, Laura; Piotrkowski, Barbara; Litterio, Maria Corina; Fraga, César Guillermo; et al.; Epicatechin protects thoracic aortic perivascular adipose tissue from whitening in high-fat fed mice; Royal Society of Chemistry; Food & Function; 11; 7; 7-2020; 5944-5954
2042-650X
CONICET Digital
CONICET
Autor
Hid, Ezequiel José
Fischerman, Laura
Piotrkowski, Barbara
Litterio, Maria Corina
Fraga, César Guillermo
Galleano, Mónica Liliana
Resumen
High adipose tissue (AT) accumulation in the body increases the risk for many metabolic and chronic diseases. This work investigated the capacity of the flavonoid (-)-epicatechin to prevent undesirable modifications of AT in mice fed a high-fat diet. Studies were focused on thoracic aorta perivascular AT (taPVAT), which is involved in the control of blood vessel tone, among other functions. Male C57BL/6J mice were fed for 15 weeks a high-fat diet with or without added (-)-epicatechin (20 mg per kg body weight per d). In high-fat diet fed mice, (-)-epicatechin supplementation: (i) prevented the expansion of taPVAT, (ii) attenuated the whitening of taPVAT (according to the adipocyte morphology, diameter, and uncoupling-protein 1 (UCP-1) levels) and (iii) blunted the increase in plasma glucose and cholesterol. The observed taPVAT modifications were not associated with alterations in the aorta wall thickness, aorta tumor necrosis factor-alpha (TNF-a) and NADPH-oxidase 2 (NOX2) expression, and endothelial nitric oxide synthase (eNOS) phosphorylation levels. In summary, our results indicate (-)-epicatechin as a relevant bioactive protecting from the slow and silent development of metabolic and chronic diseases as they are associated with excessive fat intake.