info:eu-repo/semantics/article
Local changes in chromatin accessibility and transcriptional networks underlying the nitrate response in Arabidopsis roots
Fecha
2019-09Registro en:
Alvarez, José M.; Moyano, Tomás C.; Zhang, Tao; Gras, Diana Ester; Herrera, Francisco J.; et al.; Local changes in chromatin accessibility and transcriptional networks underlying the nitrate response in Arabidopsis roots; Oxford University Press; Molecular Plant; 12; 12; 9-2019; 1545-1560
1674-2052
CONICET Digital
CONICET
Autor
Alvarez, José M.
Moyano, Tomás C.
Zhang, Tao
Gras, Diana Ester
Herrera, Francisco J.
Araus, Viviana
OBrien, José A.
Carrillo, Laura
Medina, Joaquín
Vicente-Carbajosa, Jesús
Jiang, Jiming
Gutiérrez, Rodrigo A.
Resumen
Transcriptional regulation, determined by chromatin structure and regulatory elements interacting at promoter regions, is a key step in plant responses to environmental cues. Nitrate (NO3-) is a nutrient signal that regulates the expression of hundreds of genes in Arabidopsis thaliana. Here we integrate mRNA-seq, genome-wide RNA polymerase II (RNPII), ChIP-Seq and DNase-seq data sets to establish the relationship between RNPII occupancy and chromatin accessibility in response to NO3- treatments in Arabidopsis root organs. Genomic footprinting allowed us to identify in vivo regulatory elements controlling gene expression in response to NO3- treatments. NO3--modulated TF footprints are important for a rapid increase in RNPII occupancy and transcript accumulation over time. We mapped key TF regulatory interactions and functionally validated the role of NAP, a NAC-domain containing TF, as a new regulatory factor in NO3- transport. Our strategy provides a comprehensive view of transcriptional networks in response to a nutrient signal in Arabidopsis roots.