info:eu-repo/semantics/article
A Highly Sensitive Fluorogenic Probe for Imaging Glycoproteins and Mucine Activity in Live Cells in the Near-Infrared Region
Fecha
2018-04Registro en:
Samaniego Lopez, Cecilia; Martinez, Jimena Hebe; Uhrig, Maria Laura; Coluccio Leskow, Federico; Spagnuolo, Carla Cecilia; A Highly Sensitive Fluorogenic Probe for Imaging Glycoproteins and Mucine Activity in Live Cells in the Near-Infrared Region; Wiley VCH Verlag; Chemistry- A European Journal; 24; 24; 4-2018; 6344-6348
0947-6539
CONICET Digital
CONICET
Autor
Samaniego Lopez, Cecilia
Martinez, Jimena Hebe
Uhrig, Maria Laura
Coluccio Leskow, Federico
Spagnuolo, Carla Cecilia
Resumen
A novel fluorescent molecular probe is reported, which is able to detect glycoproteins, especially mucins, with high sensitivity and with a turn-on response along with a large Stokes shift (>130 nm), within the biologically active window. The probe contains an aminotricarbocyanine as the fluorescent reporter with a linked benzoboroxole as the recognition unit, which operates through a dynamic covalent reaction between the boronic hemiester residue of the receptor and cis-diols of the analyte. The superior selectivity of the probe is displayed by the labeling of mucins present in Calu-3 cells. The new benzoboroxole fluorescent derivative gathers together key properties to make it a highly rated molecular probe: specificity, excellent solubility in water, and off–on near infrared emission. This probe is expected to be an excellent tool for imaging intracellular mucin to evaluate mucus-related diseases as well as a sensing strategy towards glycosylated structures with a high potential for theranostics approaches in biological samples.