info:eu-repo/semantics/article
Effect of the organochlorine pesticide endosulfan on GnRH and gonadotrope cell populations in fish larvae
Fecha
2011-08Registro en:
Piazza, Yanina Grisel; Pandolfi, Matias; Lo Nostro, Fabiana Laura; Effect of the organochlorine pesticide endosulfan on GnRH and gonadotrope cell populations in fish larvae; Springer; Archives of Environmental Contamination and Toxicology; 61; 2; 8-2011; 300-310
0090-4341
1432-0703
CONICET Digital
CONICET
Autor
Piazza, Yanina Grisel
Pandolfi, Matias
Lo Nostro, Fabiana Laura
Resumen
Endocrine-disrupting chemicals can influence the hypothalamus-pituitary- gonad axis and possibly affect reproduction in vertebrates. We analyzed the effect of 30-day endosulfan (ES) exposure in sexually undifferentiated larvae of the cichlid fish Cichlasoma dimerus. The number, area, mean cytoplasmic and nuclear diameter, and mean cytoplasmic optical density of gonadotropin-releasing hormone (GnRH) I, II, and III immunoreactive (ir-) neurons and β follicle-stimulating hormone (βFSH) ir-cells were measured. Animals exposed to the highest ES concentration (0.1 μg/l) showed a decrease in GnRH I nucleus/cytoplasm area ratio upon exposure. Nuclear area and mean nuclear diameter of βFSH ir-cells was higher in ES treated fish. βFSH nucleus/cytoplasm area ratio was high in exposed animals, and animals exposed to 0.1 μg/l ES showed smaller mean cytoplasmic optical density. These findings suggest that ES affects GnRH I and βFSH protein synthesis/release. However, these responses seem to be insufficient to affect gonadal differentiation at this stage of development.