publishedVersion
Bioinformatic analysis of the Type VI Secretion System and its potential toxins in the Acinetobacter genus
Autor
Repizo, Guillermo Daniel
Espariz, Martín
Seravalle, Joana L.
Salcedo, Suzana P.
Institución
Resumen
Several Acinetobacter strains are important nosocomial pathogens, with Acinetobacter
baumannii as the species of greatest concern worldwide due to its multi-drug resistance
and recent appearance of hyper-virulent strains in the clinical setting. Acinetobacter
colonization of the environment and the host is associated with a multitude of
factors which remain poorly characterized. Among them, the secretion systems (SS)
encoded by Acinetobacter species confer adaptive advantages depending on the
niche occupied. Different SS have been characterized in this group of microorganisms,
including T6SS used by several Acinetobacter species to outcompete other bacteria
and in some A. baumannii strains for Galleria mellonella colonization. Therefore, to
better understand the distribution of the T6SS in this genus we carried out an in depth comparative genomic analysis of the T6SS in 191 sequenced strains. To this
end, we analyzed the gene content, sequence similarity, synteny and operon structure
of each T6SS loci. The presence of a single conserved T6SS-main cluster (T6SS-1),
with two different genetic organizations, was detected in the genomes of several
ecologically diverse species. Furthermore, a second main cluster (T6SS-2) was detected
in a subgroup of 3 species of environmental origin. Detailed analysis also showed an
impressive genetic versatility in T6SS-associated islands, carrying VgrG, PAAR and
putative toxin-encoding genes. This in silico study represents the first detailed intra species comparative analysis of T6SS-associated genes in the Acinetobacter genus,
that should contribute to the future experimental characterization of T6SS proteins
and effectors. Para citar este articulo: Repizo GD, Espariz M,
Seravalle JL and Salcedo SP (2019)
Bioinformatic Analysis of the Type VI
Secretion System and Its Potential
Toxins in the Acinetobacter Genus.
Front. Microbiol. 10:2519.
doi: 10.3389/fmicb.2019.02519 Fil: Repizo, Guillermo Daniel. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Departamento de Microbiología; Argentina. Fil: Repizo, Guillermo Daniel. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario (IBR -CONICET); Argentina. Fil: Espariz, Martín. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Departamento de Microbiología; Argentina. Fil: Espariz, Martín. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario (IBR -CONICET); Argentina. Fil: Seravalle, Joana L. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Departamento de Microbiología; Argentina. Fil: Seravalle, Joana L. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario (IBR -CONICET); Argentina. Fil: Salcedo, Susana P. University of Lyon. Laboratory of Molecular Microbiology and Structural Biochemistry; France.