dc.contributorFrancisco Rangel Mata
dc.contributorGuadalupe Martínez Cadena
dc.contributorJuana López Godinez
dc.contributorTakuya Nishigaky
dc.contributorAlberto Darszon
dc.contributorJesús García Soto
dc.contributor0000-0002-7243-2596
dc.creatorMéndez Márquez, Rubén Octavio
dc.date.accessioned2018-01-25T19:08:35Z
dc.date.accessioned2022-10-14T15:13:43Z
dc.date.available2018-01-25T19:08:35Z
dc.date.available2022-10-14T15:13:43Z
dc.date.created2018-01-25T19:08:35Z
dc.date.issued2007-01-05
dc.identifier0006-291X
dc.identifierESSN: 1090-2104
dc.identifierhttp://hdl.handle.net/20.500.11845/479
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/4247185
dc.description.abstractAt fertilization, the sea urchin egg undergoes an internal pH (pHi) increase mediated by a Na+–H+ exchanger. We used antibodies against the mammalian antiporters NHE1 and NHE3 to characterize this exchanger. In unfertilized eggs, only anti-NHE3 cross-reacted specifically with a protein of 81-kDa, which localized to the plasma membrane and cortical granules. Cytochalasin D, C3 exotoxin (blocker of RhoGTPase function), and Y-27632 (inhibitor of Rho-kinase) prevented the pHi change in fertilized eggs. These inhibitors blocked the first cleavage division of the embryo, but not the cortical granule exocytosis. Thus, the sea urchin egg has an epithelial NHE3-like Na+–H+ exchanger which can be responsible for the pHi change at fertilization. Determinants of this pHi change can be: (i) the increase of exchangers in the plasma membrane (via cortical granule exocytosis) and (ii) Rho, Rho-kinase, and optimal organization of the actin cytoskeleton as regulators, among others, of the intrinsic activity of the exchanger.
dc.languageeng
dc.publisherElsevier
dc.relationhttp://www.sciencedirect.com/science/article/pii/S0006291X06024831
dc.relationgeneralPublic
dc.relationhttp://www.sciencedirect.com/science/article/pii/S0006291X06024831
dc.rightshttp://creativecommons.org/publicdomain/zero/1.0/
dc.rightsCC0 1.0 Universal
dc.sourceBiochemical and Biophysical Research Communications Vol. 352, Issue 1, 5 January, pp. 264–269
dc.titleRho, Rho-kinase, and the actin cytoskeleton regulate the Na+–H+ exchanger in sea urchin eggs
dc.typeArtículos de revistas


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