Tesis
Caracterização química, avaliação da toxicidade e atividade cicatrizante de feridas do extrato aquoso do infuso das folhas de Sorocea guilleminiana Gaudich.
Fecha
2018-05-29Registro en:
FIGUEIREDO, Fabiana de Freitas. Caracterização química, avaliação da toxicidade e atividade cicatrizante de feridas do extrato aquoso do infuso das folhas de Sorocea guilleminiana Gaudich. 2018. 115 f. Dissertação (Mestrado em Ciências da Saúde) - Universidade Federal de Mato Grosso, Faculdade de Medicina, Cuiabá, 2018.
Autor
Martins, Domingos Tabajara de Oliveira
http://lattes.cnpq.br/3794477872946546
Martins, Domingos Tabajara de Oliveira
109.726.923-04
http://lattes.cnpq.br/3794477872946546
Quirós, Antonio Macho
236.116.278-44
http://lattes.cnpq.br/6802168308863757
109.726.923-04
Monteiro, Karin Maia
022.013.297-61
http://lattes.cnpq.br/6742357135588288
Karuppusamy, Arunachalam
706.801.091-10
http://lattes.cnpq.br/8737379045278143
Institución
Resumen
Sorocea guilleminiana, Moraceae, popularly known as “espinheira-santa-falsa”, is a native and endemic
Brazilian shrub found in the Cerrado and Atlantic forest, whose infusion of leaves is popularly used to
treat wounds, infection, gastritis, kidney disease and inflammation. The objective of this study was to
evaluate the toxicity and wound healing activity of the aqueous extract of S. guilleminiana leaf infusion
(AESg) in experimental models in vivo and in vitro. The AESg was prepared by infusion, the leaves
powder was mixed in boiling water (10 g/L) for 15 min. Phytochemical analyzes were performed by
thin layer (TLC) and high performance liquid chromatography (HPLC). Cytotoxicity in Chinese hamster
ovary epithelial cells (CHO-K1) and cell viability of AESg in N3T3 fibroblasts (6.25 - 800 μg/mL) were
assessed by flow cytometry. The genotoxicity of AESg (10, 30 and 100 μg/mL) was evaluated in CHOK1 cells by the micronucleus (MN) and comet (COM) tests. The evaluation of AESg (0.8, 4 and 20
μg/mL) on proliferation/migration was performed in a monolayer scratch test in N3T3 cells. For acute
toxicity assessment, single oral administration of AESg (up to 2000 mg/kg) in Swiss-Webster mice was
performed. The wound healing activity of the AESg was evaluated in the excision and incision wound
models in Wistar rats. The wound healing mechanism of action of the AESg was assessed through the
evaluation of activity of myeloperoxidase (MPO) and catalase (CAT) and by the determinations of the
levels of glutathione (GSH), cytokines TNF-α, IL-1β, IL-17, IL-12p70, and IL-10 using the incision
wound model. Preliminary phytochemical analyzes of the crude extract revealed the presence of
flavonoids, steroids and terpenes. No changes were observed regarding cytotoxicity, viability, as well
as absence of genotoxicity by MN test. In the COM test, pre-treatment with AESg prevented DNA
damage at the three concentrations tested (p<0.001), while co-treatment reduced the damage only at the
lowest concentration (p<0.001). Post-treatment with AESg reduced the genetic damage induced by
hydrogen peroxide in the lowest and highest concentration (p<0.001). AESg (0.8, 4 and 20 μg/mL)
increased proliferation/migration of N3T3 cells (p<0.001). In the excision wound model, AESg (2 and
50 mg/g) increased the rate of wound contraction on the 5
th
, 7
thand 9thday (p<0.05), while Fitoscar® (60
mg/g), standard for this experiment, increased the rate of contraction at day 9 (p<0.05). There was no
change in the re-epithelialization period at any dose tested. In the incision wound AESg (2 and 50 mg/g)
was increased (p<0.01) tissue resistance to traction. AESg (2, 10 and 50 mg/g) and Fitoscar® (60 mg/g)
reduced the MPO activity (p<0.001). AESg at doses of 2 and 50 mg/g was increased levels of GSH
(p<0.05) and CAT (p<0.01). AESg did not alter the levels of TNF-α, IL-1β, IL-17 and IL-10, however,
AESg at 50 mg/g increased (p<0.05) IL-12p70 levels. It can be stated that AESg is safe when
administered orally in a single dose, does not present cytotoxicity and genotoxicity in the models tested,
and antigenotoxic potential is suggested. These results confirm the popular use of infusion of S.
guilleminiana for treatment of cutaneous wounds, probably because it stimulates the proliferation of
fibroblasts with consequent deposition of collagen and transformation in myofibroblasts are essential in
the process of wound healing, due to its antioxidant effect, possibly exerted by phenolic compounds.