Tesis
Avaliação da hiperplasia tímica e alterações imunopatológicas da medula óssea durante a infecção aguda por Leishmania amazonensis em modelo murino
Fecha
2016-10-11Registro en:
LIMA, Lusnaiara Rodrigues. Avaliação da hiperplasia tímica e alterações imunopatológicas da medula óssea durante a infecção aguda por Leishmania amazonensis em modelo murino. 2016. 52 f. Dissertação (Mestrado em Imunologia e Parasitologia Básicas e Aplicadas) - Universidade Federal de Mato Grosso, Instituto de Ciências Biológicas e da Saúde, Barra do Garças, 2016.
Autor
Souto, Paula Cristina de Souza
Silva, Wagner Welber Arrais da
http://lattes.cnpq.br/0538721794248574
http://lattes.cnpq.br/2161765071682282
Souto, Paula Cristina de Souza
277.680.138-10
http://lattes.cnpq.br/2161765071682282
Reinaque, Ana Paula Barcelos
256.830.168-60
http://lattes.cnpq.br/6456302022066024
277.680.138-10
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Jordão, Fabiana Morandi
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http://lattes.cnpq.br/7752905681664082
Institución
Resumen
Leishmaniasis is a heterogeneous group of diseases caused by protozoa of the genus
Leishmania sp. The control of infection by leishmania and dependent cell-mediated
immune response, the macrophage and the main cell effector in eliminating the
amastigote after its activation by helper T lymphocytes (helper). Lymphocytes are
produced in primary lymphoid organs, including bone marrow and thymus, bone marrow
is the production of all precursor lymphocytes and mature B cells and in the thymus T
cells mature and migrate to secondary lymphoid organs. In a recent publication, using a
murine model of infection with L. amazonensis demonstrated thymic involvement with
organ hyperplasia and derangement of architecture. Continuing the study, we analyzed
the immunopathological changes induced in the bone marrow and correlated with thymic
hyperplasia observed. Therefore, we used inbred mice of the BALB / c strain, females
aged between 6 and 8 weeks, the parasite amastigotes were inoculated in the footpad of
the hind leg, being used 28 animals divided into seven groups, control, 24 hours, 3, 5, 7,
15 and 30 days after infection. The animals were killed and the femurs and thymus
removed, the collection of peripheral blood was carried out, bone marrow aspirate,
thymus weight, Histological analysis of thymus and bone marrow, myelogram, relation
myeloid:erythroid and leukogram. Confirmed thymic hyperplasia with increased
cellularity and loss of demarcation between cortex and medulla. We observed an increase
in cellularity of bone marrow, hypoplasia of the myeloid lineage mainly myelocytes and
megakaryocytic lineage, hyperplasia of erythroid lineage characterized by increased pro-
rubrócitos and lymphoid observed on the 15th day of infection. The relation myeloid:
erythroid observed reduction cells of the myeloid lineage and increase the erythroid
lineage cells. The leucocyte count showed increased eosinophils, neutrophils and
monocytes and reduction of lymphocytes. These changes were immediately observed
after 24 hours of infection and peak occurred at 3 to 5 day. We conclude that changes in
the bone marrow may have contributed to the hyperplasia of the thymus and that the two
organs are greatly affected in the acute infection by Leishmania amazonensis.