Tesis
Padronização de esfregaço sanguíneo preparado em acetato transparente, como alternativa para o ensino do diagnóstico parasitológico da malária
Fecha
2013-12-16Registro en:
MELLO, Márcia Beatriz Cattini de. Padronização de esfregaço sanguíneo preparado em acetato transparente, como alternativa para o ensino do diagnóstico parasitológico da malária. 2013. 68 f. Dissertação (Mestrado em Ciências da Saúde) - Universidade Federal de Mato Grosso, Faculdade de Medicina, Cuiabá, 2013.
Autor
Fontes, Cor Jesus Fernandes
http://lattes.cnpq.br/5971254060419331
Fontes, Cor Jesus Fernandes
199.869.476-34
http://lattes.cnpq.br/5971254060419331
Sanchez, Bruno Antônio Marinho
047.459.696-60
http://lattes.cnpq.br/3889114548982480
199.869.476-34
Fontes, Gilberto
357.643.766-53
http://lattes.cnpq.br/5223584741856927
Institución
Resumen
Practical lessons of malaria diagnosis by microscopy is part of the
pedagogical process of academic training in medicine, biology, pharmacy and
biomedicine students, in order to qualify them for a correct malaria diagnosis in their
future practices. A good malaria practical lesson requires good teaching material,
including glass slides containing positive blood smears. Loss of glass slides is
frequently by careless students, by falling off and break them or by smear
damage. To minimize this problem, the present study was designed to describe a
methodology of preparation and validation of blood smears made on clear acetate
film sheets, in order to implementing appropriate and more easily teaching resource
available for teaching microscopic diagnosis of malaria. Several types of acetate film
were tested. Thick and thin blood smears were prepared on it and subsequently
stained by Walker and Giemsa methods, respectively. Small fragments of the stained
acetate were cut and mounted onto glass slides for further microscopic
examination. The evaluation of the quality of the new method was made by
comparing the results of microscopic observation image and parasite density,
performed by 3 microscopists. The study showed that transparent acetate films are
feasible for making thick and thin blood smears to diagnosis malaria by microscopy.
This is a simple and low cost technical implementation that does not compromise on
morphological and tinctorial features of parasites nor of the blood cells into the
smears. Because it is lightweight and flexible characteristics, the acetate film has the
advantage of easy handling and transport. In addition, the acetate fragments
containing stained blood smears can be reused if the glass slides breaks.