Aplicação da técnica de Análise de Diferença Representacional (RDA) para identificação de genes diferencialmente expressos em Conidiobolus lamprauges cultivados a 30 e 37ºc

Abstract

The Conidiobolus lamprauges is a zygomycetes fungi pathogen of humans and animals, responsible for conidiobolomycosis, which is characterized by a severe granulomatous chronic rhinosinusitis. This serious disease is endemic in ovines in the Northeast region of the country, and it has being diagnosed frequently in the State of Mato Grosso. The ability to adapt and to grow at high temperatures is suggested as an attribute of virulence in fungi that infect animals and humans, however regarding C. lamprauges little information is available about this aspect. This paper aims at identifying differential expression genes in C. lamprauges obtained from sick ovines. The fungi was grown on Sabouraud Dextrose 2%, at temperatures of 30° and 37° C, and, after extracting RNA, two subtracted libraries were built, of which was obtained a set of 120 cDNAs which, after the analysis, enabled the identification of a glycolytic enzyme called enolase differentially expressed at 37°C, and this enzyme performs functions related to pathogenicity and to host-pathogen interaction process in several pathogenic microorganisms, showing a potential involvement in host-pathogen relationship, and virulence in C. lamprauges, demonstrating the feasibility of RDA technique to analyze transcripts of C. lamprauges owing to the temperature