Tesis
Caracterização molecular, clínica, patológica e econômica de um surto de mortalidade em bezerros por “Hobi-like virus”, Brasil
Fecha
2013-02-20Registro en:
CRUZ, Raquel Aparecida Sales da. Caracterização molecular, clínica, patológica e econômica de um surto de mortalidade em bezerros por “Hobi-like virus”, Brasil. 2013. 68 f. Dissertação (Mestrado em Ciências Veterinárias) - Universidade Federal de Mato Grosso, Faculdade de Agronomia, Medicina Veterinária e Zootecnia, Cuiabá, 2013
Autor
Colodel, Edson Moleta
http://lattes.cnpq.br/6150957874684255
Colodel, Edson Moleta
766.442.439-91
http://lattes.cnpq.br/6150957874684255
Nakazato, Luciano
638.389.071-91
http://lattes.cnpq.br/3898850578198054
766.442.439-91
Lunardi, Michele
046.370.609-90
http://lattes.cnpq.br/9832458184144869
Institución
Resumen
"Hobi-like virus" or atypical pestivirus has been detected in animals infected
naturamente on three continents, but the clinical disease associated with infection
Hobi-like virus has been little studied. In Brazil, the virus was associated with bovine
abortion and respiratory lesions in Italy. In this paper we report the epidemiological,
clinical, pathological, molecular and economics of an outbreak of infection Hobi-like
pestivirus in calves with diarrhea in a property located in the city of Cuiabá / MT in
2010. During the outbreak killed 20 calves on the property, which was possible to
follow three animals. The first calf was referred to the Veterinary Hospital of UFMT for
necropsy. Two other calves (2:03) were monitored in HOVET / UFMTe were
necropsied after death. Regarding the clinical history, the first calf showed signs of
apathy, dehydration and diarrhea yellow for 3 days and at necropsy yellowish
contents in the intestine, and peritonitis omphalophlebitis. 2:03 calves showed diffuse
alopecia on the back, epidermal hyperkeratosis, ulcers and erosions covered with
plates of fibrin. Microscopic examination of the affected tissues showed
omphalophlebitis and suppurative peritonitis in 1 Calf and extensive areas of necrosis
(ulcers and erosions) by various organs of calves 2:03. For virological diagnosis were
sampled for blood and lymphoid organs of calves 2 and 3, which were processed and
subjected to RT-PCR for BVDV. Products of amplification of the 5 'untranslated
region and viral nucleoprotease obtained were purified and sequenced. Phylogenetic
analyzes of the sequences now denonimada sample LPV-WR/BR11 allowed their
classification in the group of atypical pestivirus. Immunohistochemistry for BVDV
revealed positive staining for viral protein mainly in epithelial cells of the skin, oral
cavity and the organs of the digestive system. We estimated a direct cost of the
disease in the herd of R $ 22,500.00. The data presented demonstrate that this
characterization Hobi-like viruses can cause calf mortality for acute gastroenteritis
associated dermatitis in suckling calves.