Tesis
Identificação de transcritos diferencialmente expressos por Pasteurella multocida em condição de privação de ferro por sequenciamento de nova geração
Fecha
2015-08-07Registro en:
SILVA, Mayara Inácio Vincenzi da. Identificação de transcritos diferencialmente expressos por Pasteurella multocida em condição de privação de ferro por sequenciamento de nova geração. 2015. 39 f. Dissertação (Mestrado em Ciências Veterinárias) - Universidade Federal de Mato Grosso, Faculdade de Agronomia, Medicina Veterinária e Zootecnia, Cuiabá, 2015.
Autor
Dutra, Valéria
Nakazato, Luciano
http://lattes.cnpq.br/3898850578198054
http://lattes.cnpq.br/4478191386305454
Dutra, Valéria
501.674.720-20
http://lattes.cnpq.br/4478191386305454
Pacheco, Richard de Campos
791.476.071-49
http://lattes.cnpq.br/5213594247690553
501.674.720-20
638.389.071-91
Oliveira Filho, João Xavier de
975.819.921-87
http://lattes.cnpq.br/6020939263664420
Pescador, Caroline Argenta
958.659.180-87
http://lattes.cnpq.br/5754349416478829
Institución
Resumen
Iron (Fe) is an essential element and the ability to acquire it, in vivo, have been described in several pathogens such as virulence factors. Global analyses of transcripts during iron deprivation have been described by microarray studies, however, recently RNA-seq analysis shows superior results. The high pathogenic swine strain of Pasteurella multocida (BRMSA 1113) was grown in the two conditions with different concentrations of Fe (control and deprivation) in order to analyze the differentially expressed transcripts. The total RNA of the two conditions was extracted and sequenced by new generation Ion Torrent plataform. Data were analyzed in Ion Reporter ™ Software and processed in Rockhopper software. Sequence analysis shows 1,341,615 readings with median length of 81pb, with 96% of alignment to the reference genome Pasteurella multocida strain 3489, and 98.8% accuracy. Reads mapping to genome of P. multocida in these two conditions, detected 2,652 transcripts, which , 177 (6.7%) were differentially expressed, with 93 in the control condition (Fe +) and 84 provided with iron deprivation condition (Fe-). In condition (Fe-), differential expressed transcript profile were associated to function of cellular transport (fbpABC,, high-affinity Fe2+/Pb2+ permease and periplasmic protein probably involved in hight-affinity Fe2+), transcptional regulators and hypothetical proteins. The control condition (Fe+) shows differential expressed transcripts profile associated to RNA anti-sense (asRNA) energetic metabolism genes (fructose-1,6-bisphosphatase). The technique of RNA-seq proved effective in identifying novel differentially expressed transcripts by P. multocida (BRMSA 1113) under conditions of Fe deprivation, which can assist in pathogenicity studies.