dc.creator | TORRES V,PABLO | |
dc.creator | SEPÚLVEDA C,M. JACQUELINE | |
dc.creator | VON PLESSING R,CARLOS | |
dc.date | 2011-01-01 | |
dc.date.accessioned | 2017-03-07T16:35:44Z | |
dc.date.available | 2017-03-07T16:35:44Z | |
dc.identifier | http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-97072011000100019 | |
dc.identifier.uri | http://repositorioslatinoamericanos.uchile.cl/handle/2250/404301 | |
dc.description | A new, simplified solid phase extraction procedure for the determination of risperidone and 9-hydroxyrisperidone in human plasma has been developed. This method involves the use of an optimized extraction protocol developed in Waters OASIS® HLB 30mg 1cc extraction columns using 1 mL of human serum. Separation was performed by HPLC using a Waters XTerra RP-18 (5 µm, 150x4,6 mm) column with a mobile phase consisting in acetonitrile - potassium dihydrogen phosphate 50 mM pH 3.4 (27/73). UV detection at 278 nm was used to quantify analytes, encountering good linearity (r² > 0.999) in the 2-100 ng/mL concentration range. The mean recovery was 92.4 % and 92.8 % for risperidone and 9-hydroxyrisperidone respectively, with an intraday - interday precision below 7%, and accuracy below 115 %. The method has been successfully applied in pharmacokinetic studies that require a large sample number. | |
dc.format | text/html | |
dc.language | en | |
dc.publisher | Sociedad Chilena de Química | |
dc.source | Journal of the Chilean Chemical Society v.56 n.1 2011 | |
dc.subject | Risperidone | |
dc.subject | 9-hydroxyrisperidone | |
dc.subject | solid phase extraction | |
dc.title | PHARMACOKINETIC STUDY OF RISPERIDONE: APPLICATION OF A HPLC METHOD WITH SOLID PHASE EXTRACTION | |
dc.type | Artículos de revistas | |