Dissertação
Efeito da rutina e da curcumina em marcadores inflamatórios e oxidativos em modelo experimental de hiperlipidemia
Fecha
2019-02-28Autor
Manzoni, Alessandra Guedes
Institución
Resumen
Hyperlipidemia is characterized by lipid abnormalities that result in inflammation and
oxidative damage, factors involved in the development of atherosclerosis. Adenosine and
adenosine deaminase (ADA) modulate immune and inflammatory responses as well as lipid
metabolism. Curcumin and rutin are polyphenols with antioxidant, anti-inflammatory, and
hypolipidemic potential. Thus, the objective of this study was to evaluate the activity of ADA
in lymphocytes, platelets, neutrophils, and serum and the oxidative profile in liver and kidney
of rats with induced hyperlipidemia, treated with curcumin and/or rutin. Adult Wistar male rats
were pre-treated by gavage with rutin and/or curcumin for 30 days. Hyperlipidemia was acutely
induced by intraperitoneal administration of Poloxamer-407 (P-407). After 36 hours, the
animals were euthanized, and blood was collected for hemogram and serum and cell separation
to evaluate enzymatic activity and biochemical parameters. The liver and kidney were collected
to evaluate oxidative stress markers and antioxidant defense. P-407 induction increased levels
of total cholesterol and triglycerides, reactive oxygen species (ROS), ADA and
myeloperoxidase activity (MPO), and markers of oxidative damage in the liver, reduced
antioxidant defenses and caused hematological changes. The pre-treatments prevented the
increase in triglycerides, plasma MPO activity, ADA activity in serum and cells, oxidative
stress in the liver and kidney, prevented the reduction of high-density lipid levels and
antioxidant defenses in the liver and kidney, as well as reversing hematological parameters.
Thus, positive effects of curcumin and/or rutin pre-treatments on the effects of hyperlipidemia
were demonstrated, suggesting that their use as adjuvants may benefit patients with
hyperlipidemia.