Dissertação
Micotoxinas no milho roxo peruano
Fecha
2017-09-29Autor
Adaniya, Zoila Naeko Coloma
Institución
Resumen
Purple corn is a variety of corn that is produced in the Peruvian Andes. Its characteristic color
is by the presence of the pigment called antiocyanine that presents antioxidant, antimutagenic,
anticancer and anti-diabetic properties. These characteristics have made that in addition to
being used as a dye, is used as raw material in the elaboration of several by-products. Currently
Peru exports this input, however, due to the lack of regulation and because it is considered an
organic product, mycotoxin analyzes are not required. These toxins can be produced by
filamentous fungi at any stage of the maize food chain. Its consumption by humans or animals
can cause carcinogenic, mutagenic, hepatotoxic, estrogenic, immunotoxic and nephrotoxic
effects, which is why international organisms have established maximum tolerable limits
(MTL) of mycotoxins in foods to try to control exposure. Considering the possible effects of
mycotoxins on consumers of Peruvian purple maize, the objective was to determine the
presence of mycotoxins in this corn through LC-MS/MS. A total of 82 samples of purple
maize were obtained from different Peruvian markets in two periods: December 2015 to
March 2016 and March to April 2017. The mycotoxins analyzed were aflatoxins, fumonisins,
zearelenone, ochratoxin A and the following trichothecenes: deoxynivalenol, nivalenol,
fusarenone X, deacetoxyscirpenol, 3-acetyl-DON, HT-2 and T-2. It was verified that the most
prevalent mycotoxins were aflatoxins and fumonisins, with a prevalence of 64.6 and 63.4%,
respectively, with co-occurrence of these two mycotoxins in 45.1% of the samples. Only one
sample had a quantifiable concentration for zearalenone and any sample was contaminated by
ochratoxin A and trichothecenes. Considering the MTLs implemented by the European
Community (EC, 2006, 2007, 2013), 12% of the analyzed samples had concentrations higher
than the MTL of 1000 μg kg-1 for fumonisins and one sample had MTL of 10 μg kg-1 higher
for aflatoxins. However, considering the Brazilian legislation, 9.8% of the samples had levels
above the MTL of 5000 μg kg-1 of fumonisins (BRASIL, 2011, 2013, 2017). This is the first
research that evaluates the occurrence of mycotoxins analyzed by LC-MS/MS in Peruvian
purple maize and identifies that maize may constitute a source of intoxication, posing a risk
to public health. Controls are required in the production chain of this grain and the
implementation of legislation with MTLs of mycotoxins.