Divergent effects of Nitric oxide on airway epithelial cell activation

dc.creatorNeri,Tommaso
dc.creatorConti,Ilaria
dc.creatorCerri,Chiara
dc.creatorTavanti,Laura
dc.creatorPaggiaro,Pierluigi
dc.creatorCeli,Alessandro
dc.date2010-01-01
dc.date.accessioned2017-03-07T16:32:22Z
dc.date.available2017-03-07T16:32:22Z
dc.identifierhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602010000400012
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/403230
dc.descriptionNitric oxide (NO*) is a gaseous mediator synthesized by Nitric oxide sinthases. NO* is involved in the modulation of inflammation, but its role in airway inflammation remains controversial. We investigated the role of NO* in the synthesis of the chemok Nes Interleukin-8 and Monocyte Chemotactic Protein-1, and of Intercellular Adhesion Molecule-1 by human airway epithelial cells. normal human bronchial epithelial cells and the bronchial epithelial cell line BEAS-2B were used. Neterleukin-8 (IL-8) and Monocyte Chemotactic Protein-1 (MCP-1) secretion and Intercellular Adhesion Molecule-1 (ICAM-1) expression were measured by ELISA. mRNA was assessed by semiquantitative RTI-PCR. Neterleukin-8 secretion was significantly reduced after 24h incubation with the NO* donor, sodium nitroprusside. The effect was dose-dependent. Similar results were obta Ned with S-Nitroso-N-D,L-penicillam Ne and S-Nitroso-L-glutathione. Inhibition of endogenous NO* with the Nitric oxide synthase inhibitor N-Nitro-L-arg N Ne-methyl-esther caused an increase in IL-8 secretion by lypopolisaccharide- and cytok Ne-stimulated BEAS-2B cells. Sodium nitroprusside also caused a reduction in Monocyte Chemotactic Protein-1 secretion by both cell types. In contrast, Intercellular Adhesion Molecule-1 expression was upregulated by sodium NItroprusside. RTI-PCR results indícate that the modulation of protein levels was paralleled by modification in mRNA levels. NO* has divergent effects on the synthesis of different inflammatory mediators in human bronchial epithelial cells.
dc.formattext/html
dc.languageen
dc.publisherSociedad de Biología de Chile
dc.sourceBiological Research v.43 n.4 2010
dc.subjectAdhesion molecules
dc.subjectAirway inflammation
dc.subjectAlveolar Epithelium
dc.subjectChemok Nes
dc.subjectNitric Oxide
dc.titleDivergent effects of Nitric oxide on airway epithelial cell activation
dc.typeArtículos de revistas


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