Artigo de Periódico
Solid–Liquid Equilibrium of Substrates and Products of the Enzymatic Synthesis of Ampicillin
Fecha
2010Registro en:
0001-1541
v. 56, n. 6
Autor
Santana, Mônica Leila Portela de
Ribeiro, Marcelo P. A.
Leite, Geisa A.
Giordano, Raquel L. C.
Giordano, Roberto C.
Silva, Silvana Mattedi e
Santana, Mônica Leila Portela de
Ribeiro, Marcelo P. A.
Leite, Geisa A.
Giordano, Raquel L. C.
Giordano, Roberto C.
Silva, Silvana Mattedi e
Institución
Resumen
The solid–liquid equilibrium of precursors and products of the enzymatic synthesis
of ampicillin (AMP) [6-aminopencillanic acid (6-APA) and D( )phenylglycine (PG)]
was investigated at different temperatures (283–298 K) and pHs (5.5–7.5). Solubility
data were obtained using an analytical methodology. Equilibrium dissociation constants
were experimentally measured at several temperatures for AMP, 6-APA, PG,
and D( )phenylglycine methyl ester. A model based on the simplified perturbed hard
sphere theory proposed by Khoshkbarchi and Vera (Ind Eng Chem Res. 1996;35:4319-
4327) was fitted against solubility data. The model could describe the water solubility
behavior for AMP and PG as function of pH and temperature, but a bias was observed
when fitting the model to the solubility of 6-APA. VVC 2009 American Institute of Chemical
Engineers AIChE J, 56: 1578–1583, 2010