Efeito da cafeína na movimentação ortodôntica e no comportamento de ratos: estudo experimental in vivo

Abstract

OBJECTIVE: To verify the effect of caffeine on orthodontic movement (MO) and behavior of rats. METHODS: In this randomized in vivo experimental study, 12 healthy male Wistar rats (Rattus norvegicus albinus), 7-12 weeks, 200-300g were used. These animals were submitted to OM (closed NiTi spring (50cN) between 1st molar and upper incisors / left side) and daily administration of caffeine (3g / L) and water by gavage for 21 consecutive days. The sample was divided into two groups: a) control (n = 7): submitted to OM and water; b) experimental (n = 5): submitted to OM and caffeine. The amount of OM was verified by a dry point compass and millimeter ruler at the beginning and end times. The open field test was used in the four-stage behavioral assessment: baseline (T0), after spring placement (T1), after 2nd gavage (T2), after 21 days (T3). Then, Mann-Whitney statistical tests (amount of OM) and Friedman test / Wilcoxon test (behavior) were performed at a significance level of 5%. RESULTS: Caffeine did not interfere with OM (p> 0.05). Regarding the open field test, statistically significant values (p> 0.05) were identified in several parameters. CONCLUSION: Caffeine did not influence the amount of macroscopic orthodontic movement, despite exhibiting anxiogenic effect on rat behavior.