masterThesis
Efeitos da suplementação de creatina no estado redox do tecido renal de ratos diabéticos induzidos por estreptozotocina
Fecha
2019-03-14Registro en:
MEDEIROS, Matheus Anselmo. Efeitos da suplementação de creatina no estado redox do tecido renal de ratos diabéticos induzidos por estreptozotocina. 2019. 45f. Dissertação (Mestrado em Bioquímica) - Centro de Biociências, Universidade Federal do Rio Grande do Norte, Natal, 2019.
Autor
Medeiros, Matheus Anselmo
Resumen
Introduction: Creatine is a nutritional supplement that has been the subject of research
in several pathologies, with a wide possibility of clinical applications. Studies have shown
the effect of creatine on increasing glucose uptake by elevating the expression of GLUT4 receptors in metabolic disorders such as type II Diabetes Mellitus, however, there are
no studies that report the effect of creatine on the glycemic metabolism of type I Diabetes
Mellitus. Thus, this work aims to evaluate the effect of creatine supplementation in
diabetic rats induced by streptozotocin. Methods: Thirty-two Wistar rats were divided
into four groups: (C) animals without diabetes and without creatine supplementation (n =
8), (CCr) animals without diabetes and creatine supplementation (n = 8), (D) animals
Streptozotocin-induced diabetics without supplementation (n = 8), (DCr) creatine-treated
diabetic-induced diabetic animals (n = 8). Groups D and DCr received a single dose of
streptozotocin (40 mg/kg i.p.). The following evaluations were performed: clinical,
biochemical, as well as the analysis of redox state parameters in the renal tissue of the
animals. Results and discussion: In fasting glycemia, diabetic groups presented
significant hyperglycemia in comparison with the control groups, but the DCr group
showed a significant improvement in relation to group D. This result corroborates
previous studies that verified the same positive effect in pathological conditions that
culminate in hyperglycemia. In the quantification of urea and alanine transaminase, the
diabetic groups presented a significant increase in comparison with the control groups,
however, the DCr group showed a significant improvement in relation to group D in both
parameters. Our results of ALT and serum urea in group D can be interpreted as an
increase in systemic gluconeogenesis, provable hepatic injury and protein catabolism,
since urea is the main product of the ornithine cycle. The enzymatic activity of catalase,
superoxide dismutase and glutathione peroxidase were significantly lower in group D
compared to control groups, however, group DCr had restored activity at similar level to
the control groups in all enzymes. In the quantification of the hydrogen peroxide content,
group D had significantly higher levels in comparison to the control groups, however,
group DCr had a similar level of restoration to the control groups in this parameter. These
results converge with previous studies that observed that creatine supplementation
modulates the enzymatic antioxidant activity and may be a direct antioxidant agent of
reactive oxygen species. Conclusion: Creatine can improve metabolic profile in DM and
restore redox state parameters in this condition. These results support creatine
supplementation as a potential adjunctive therapeutic agent.