SIMULTANEOUS DETERMINATION OF GLUTATHIONE AND GLUTATHIONE DISULFIDE IN AN ACID EXTRACT OF PLANT SHOOT AND ROOT BY CAPILLARY ELECTROPHORESIS

dc.creatorMENDOZA,JORGE
dc.creatorGARRIDO,TATIANA
dc.creatorRIVEROS,RAÚL
dc.creatorGONZÁLEZ,CECILIA
dc.creatorPARADA,JOSÉ
dc.date2008-09-01
dc.date.accessioned2017-03-07T16:02:46Z
dc.date.available2017-03-07T16:02:46Z
dc.identifierhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-97072008000300018
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/395142
dc.descriptionThis study describes the fast and simultaneous determination of glutathione and glutathione disulfide by Capillary Zone Electrophoresis in plant extracts of shoot and root of tomato plants. Frequentuse of acidic precipitation of protein generates an acidic matrixof strength and pH tha may cause changes inthe method sensitivity, comigration of species or changes in the equilibria that relate both species in cells or fluids. In this study, the resulting acidic matrix was previously treated with the same background electrolyte to prevent comigration and to improve signal resolution. Optimization of some parameters of the technique allowed the determination of both analytes in less than three minutes. The optimized method showed good reproducibility and linearity, with correlation coefficients above 0.999 and detection limits below 3 µM for both peptides. Analyte recovery in the process was in the 88-104% range. The concentration found in tomato plants hydroponically grown inthe absence of stress factors was inthe 51-100 nmol g-1 range, fresh weight for GSH and 5-32 nmol g-1 range, fresh weightfor GSSG
dc.formattext/html
dc.languageen
dc.publisherSociedad Chilena de Química
dc.sourceJournal of the Chilean Chemical Society v.53 n.3 2008
dc.subjectGlutathione
dc.subjectCapillary Electrophoresis
dc.subjectMetaphosphoric acid
dc.subjectAcidic precipitation
dc.subjectGlutathione disulfide
dc.titleSIMULTANEOUS DETERMINATION OF GLUTATHIONE AND GLUTATHIONE DISULFIDE IN AN ACID EXTRACT OF PLANT SHOOT AND ROOT BY CAPILLARY ELECTROPHORESIS
dc.typeArtículos de revistas


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