SPECTROPHOTOMETRIC STUDIES ON THE INTERACTION OF YEAST RNA WITH CRYSTAL VIOLET AND ITS ANALYTICAL APPLICATION

dc.creatorZHAO,NA
dc.creatorWANG,MANXIA
dc.creatorNIU,XUELIANG
dc.creatorSUN,WEI
dc.creatorJIAO,KUI
dc.date2008-09-01
dc.date.accessioned2017-03-07T16:02:43Z
dc.date.available2017-03-07T16:02:43Z
dc.identifierhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-97072008000300009
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/395133
dc.descriptionThe interaction of crystal violet (CV) with yeast RNA (yRNA) was studied by UV-Vis absorption spectrophotometric method in this paper and further a simple spectrophotometric method for the yRNA determination was proposed. At a pH 4.0 Britton-Robinson (B-R) buffer solution, CV had a máximum absorption peak at 590 nm, which was greatly decreased by the addition of yRNA. The conditions of CVinteracted with yRNA were carefully investigated. Underthe optimal conditions the decrease of absorbance value was proportional to the concentration of yRNA in the range from 3.33x10-6 to 1.67x10-5 mol L-1 when 2.0 x 10(5) mol L-1 CV was employed. The detection limit was calculated as 5.34 x 10-7 mol L-1 (3σ) and different samples were determined satisfactorily. The binding number and the binding constant of CV with yRNA were calculated by spectrophotometric data with the results as 1 and 1.69 x 10(5) L mol-1, respectively. The reaction mechanism was discussed with the electrostatic attraction led to molecular association of yRNA with CV
dc.formattext/html
dc.languageen
dc.publisherSociedad Chilena de Química
dc.sourceJournal of the Chilean Chemical Society v.53 n.3 2008
dc.subjectcrystal violet
dc.subjectyeast RNA
dc.subjectUV-Vis spectrophotometry
dc.subjectinteraction
dc.titleSPECTROPHOTOMETRIC STUDIES ON THE INTERACTION OF YEAST RNA WITH CRYSTAL VIOLET AND ITS ANALYTICAL APPLICATION
dc.typeArtículos de revistas


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